Responses of Root Growth to Moderate Soil Water Deficit in Wheat Seedlings

Abstract

In the field, plants show better root growth in drying soil than in wet soil. However, the root growth enhancement has not been demonstrated clearly in the laboratory. In this study, the root growth response of wheat seedlings to moderate soil water deficits was characterized quantitatively in an environment-controlled chamber. Germinated seeds of wheat were grown for 15 days in the soil with a water potential ranging from field capacity (FC) to approximately –0.08 MPa. Theleaf area decreased with reduction in soil water potential. By contrast, the root surface area increased upon reduction ofthe soil water potential to –0.04 MPa while it decreased significantly in soil with a water potential of –0.08 MPa. The increase in surface area was obvious in the roots with a diameter of 0.2 to 0.4 mm and larger than 0.7 mm. Root weight increased with the reduction of soil water potential to –0.04 MPa. While specific root length decreased significantly with the reduction of water potential to –0.06 MPa, the specific root surface area did not. Assimilatestransported from shoot might be used in roots to increae the surface area mainly by increasing the diameter rather than the length in response to a moderate soil water deficit in wheat seedlings. This might result from the drought tolerance mechanism of osmotic adjustment in roots.     

Influence of Day Length on Stem Growth,Flowering, Morphology of Flower Clusters,and Seed-Set in Buckwheat (Fagopyrum esculentum Moench)

Abstract

The effects of day length on main stem growth, flowering, morphology of flower clusters and seed-set were examined in three buckwheat cultivars Shinanonatsusoba (summer eco-type), Miyazakizairai (autumn eco-type) and BLO 1999 (a long cluster line which usually develops DM clusters at Kade Research Ltd., Canada). Long-day treatment prolonged the stem elongation period, elevated the first flowering node, delayed the first flowering day, increased the numbers of nodes, flower clusters and flowers on the main stem, and decreased the increase rate of flowering-cluster number, the number of seeds and the seed-set ratio on the main stem. It also increased the frequency of DM clusters, the length of the flower clusters and the number of sub-flower-clusters per cluster in Shinanonatsusoba and Miyazakizairai as well as in BLO 1999. The effects of day length varied among the growth parameters and there were three types of responses to day length. The difference between the summer and autumn eco-type cultivars in the responses to day length was elucidated in four groups of parameters; (1) main stem elongation; (2) first flowering node and first flowering day; (3) increase rate of flowering-cluster number on the main stem; and (4) the number of seeds and seed-set ratio.     

鲤头长、体厚、体高性状的QTL定位及遗传效应分析

用174个SSR、41个EST、345个SNP标记对以镜鲤良种后代为祖父母本所培育的杂交F2群体的68个个体进行基因型检测,运用JoinMap4.0软件包构建遗传连锁图。利用MapQTL5.0区间作图法(interval mapping,IM)和多QTL区间定位法(MQM mapping,MQM)进行QTL检测,通过置换实验(1000次重复)确定连锁群显著性水平阈值。在对体高、头长、体厚的区间定位中,共检测到6个与体高性状相关的QTLs区间,分布在LG1(SNP1339-SNP1490)、LG10(HLJE469-SNP1491)、LG12(SNP0922-HLJ1316)、LG13(SNP0937-HLJ328)、LG25(SNP1041-HLJ594)、LG35(SNP1425-SNP0389)等6个连锁群上,解释表型变异范围为20.0%～43.3%。其中,SNP1339-SNP1490区间LOD值最大为3.64,解释表型变异35.4%。6个与头长相关的QTLs,分布在LG1(SNP1339-SNP1490)、LG12(HLJ071-HLJ336)、LG13(SNP0937-HLJ328)、LG24(SN...     

Evaluating grassed waterway efficiency in southeastern Iowa using WEPP

Properly calibrated, single storm event, model simulations can be valuable quantitative tools for evaluating the effectiveness of grassed waterways (GWWs) and identifying a threshold length for an effective GWW. Current specifications for GWW lengths in Iowa are lacking. The key objective of this study is to employ the well-established Water Erosion Prediction Project model (WEPP) for determining an effective (or threshold) GWW length for reducing runoff and sediment yields under a wide range of hydrologic and management conditions in an agricultural Iowa watershed, namely Clear Creek, IA. The advantage of this physically based, distributed-parameter model is its ability to replicate processes at the hillslope scale where GWW performance is mostly evaluated. Overall, 84 WEPP runs were performed for assessing (i) the effect that GWW length has on reducing runoff and sediment yields within a representative test hillslope, and (ii) the effect of the gradient of the drainage area on GWW efficiency. Results show that the GWW efficiency for all GWW lengths is governed by hydrology, expressed as Q_peak. The results suggest that the threshold length for an effective GWW is 500 m for the hydrologic conditions in this study and for a representative drainage area of approximately 27 ha. For all storm events, a threshold drainage area gradient of approximately 3% was found above which the GWW efficiency was independent of topographic steepness. The results demonstrate similar trends to other studies although the relative increases in reduction in runoff and sediment delivery differ between sites and are very much dictated by Q_peak and hydrologic soil group. The effectiveness of models to evaluate GWW efficiency for nearly saturated conditions and shallow flows is discussed.     

中国部分地区绵羊肺炎支原体的基因多态性分析

为了解绵羊肺炎支原体(Mycoplasma ovi pneumoniae,Mo)在我国部分地区的流行病学特征,采用随机扩增多态性DNA(RAPD)及限制性片段长度多态性(RFLP)方法对26株Mo基因多态性进行了研究,并利用NTsys2.10e软件对获得的多态性图谱进行了聚类分析.结果在相似系数为0.70时,26株Mo可分成6个RAPD群或6个RFLP群;相似系数为0.90时,分成18个RAPD群或18个RFLP群;相似系数为1.00时,分成25个RAPD群或26个RFLP群.结果表明,我国Mo存在高度的基因多态性,这种多态性与地域差异相关,与宿主来源也具有一定的相关性.研究结果为了解我国Mo的分子流行病学特征打下了基础,也为建立有效的Mo诊断方法和疫苗研制提供了参考依据.     

十个芍药品种气孔特性研究

选取10个品种的芍药作为研究对象,观察芍药叶片气孔的形态、分布规律,并对其气孔大小、气孔长宽比、表皮细胞密度、气孔密度及气孔指数等进行品种间比较及差异分析.结果表明:10个品种的芍药叶片气孔形态及分布规律一致.各项指标的品种内变异系数均较小.4个品种(1、2、8、9)的4项指标(气孔长度、宽度、长宽比及气孔指数)均没有差异.2个品种(1、9)的5项指标(气孔长度、宽度、长宽比、气孔密度及气孔指数)均没有差异.各项指标相比较,气孔宽度和气孔指数在各品种间变化较小.气孔密度与气孔长度呈反相关.结果不支持将气孔长度、宽度、气孔长宽比、气孔密度、气孔指数及表皮细胞密度作为芍药种或品种分类的依据.     

基于ASTRE和SRTM高程数据的坡度和坡长提取与分析

为了使SRTM和ASTER高程数据得到科学利用,选择东北漫岗丘陵区和黄土丘陵区,基于网络下载的SRTM和ASTER高程数据,利用数字地形分析的标准方法进行了数据处理。提取了坡度和坡长两个地形指标并与Hc-DEM(水文地貌关系正确的数字高程模型)及其提取的地形参数进行了对比,结果表明:(1)无论是高程、坡度还是坡长,均是Hc-DEM系列的数据表现最好,SRTM次之,ASTER表现较差;(2)在地形平坦地区,ASTER表面因明显的空值和洼地,使高程及其基础上提取的地形参数不具实用价值,但在地形比较陡的地区则可用;(3)基于SRTM和Hc-DEM提取的两组坡度和坡长具有相同的格局,但基于SRTM的坡度有衰减,坡长有扩展,实际应用前需进行尺度变换。     

基于激光三角法的地表粗糙度测试仪的研制

针对接触法测试地表粗糙度需要人工参与、测试时间长等问题,该文提供了一种快速的地表粗糙度测试的仪器及测试方法。基于激光三角原理,将激光投影到待测目标上,摄像机接收反射光的图像,通过图像处理算法及坐标转换得到被测区域的三维坐标,再根据粗糙度计算公式得到地表粗糙度参数的结果。将该仪器分别进行了精度及野外测试试验,精度试验测量结果的平均相对误差最大值为2.93%,仪器典型分辨率在垂直仪器扫描方向为0.78 mm,仪器扫描方向为1 mm,垂直大地方向为0.83 mm。试验结果表明,农田表面空间自相关系数的测量结果反映出了农田的垄结构特征,验证了该文仪器及方法的可行性及有效性。     

Phosphate Solubilizing Ability and Phylogenetic Diversity of Bacteria from P-Rich Soils Around Dianchi Lake Drainage Area of China

The phylogenetic diversity of phosphate solubilizing bacteria(PSB)distributed in P-rich soils in the Dianchi Lake drainage area of China was characterized,and the tricalcium phosphate(TCP)solubilizing activities of isolated PSB were determined.Among 1 328 bacteria isolated from 100 P-rich soil samples,377 isolates(28.39%of the total)that exhibited TCP solubilization activity were taken as PSB.These PSB showed different abilities to solubilize TCP,with the concentrations of solubilized P in bacterial cultures varying from 33.48 to 69.63 mg L^-1.A total of 123 PSB isolates,with relatively high TCP solubilization activity(>54.00 mg L^-1),were submitted for restriction fragment length polymorphism(RFLP)analysis,which revealed 32 unique RFLP patterns.Based on these patterns,62 representative isolates,one to three from each RFLP pattern,were seffected for 16S rRNA sequencing.Phylogenetic analysis placed the 123 PSB into three bacterial phyla,namely Proteobacteria,Actinobacteria and Firmicutes.Members of Proteobacteria were the dominant PSB,where 107 isolates represented by 26 RFLP patterns were associated with the genera of Burkholderia,Pseudomonas,Acinetobacter,Enterobacter,Pantoea,Serratia,Klebsiella,Leclercia,Raoultella and Cedecea.Firmicutes were the subdominant group,in which 13 isolates were affiliated with the genera of Bacillus and Brevibacterium.The remaining 3 isolates were identified as three species of the genus Arthrobacter.This research extends the knowledge on PSB in P-rich soils and broadens the spectrum of PSB for the development of environmentally friendly biophosphate fertilizers.     

在富营养土壤斑块中根增值对玉米养分吸收和生长的贡献

Root proliferation can be stimulated in a heterogeneous nutrient patch; however, the functions of the root proliferation in the nutrient-rich soil patches are not fully understood. In the present study, a two-year field experiment was conducted to examine the comparative effects of localized application of ammonium and phosphorus (P) at early or late stages on root growth, nutrient uptake, and biomass of maize (Zea mays L.) on a calcareous soil in an intensive farming system. Localized supply of ammonium and P had a more evident effect on shoot and root growth, and especially stimulated fine root development at the early seedling stage, with most of the maize roots being allocated to the nutrient-rich patch in the topsoil. Although localized ammonium and P supply at the late stage also enhanced the fine root growth, the plant roots in the patch accounted for a low proportion of the whole maize roots in the topsoil at the flowering stage. Compared with the early stage, fine root length in the short-lived nutrient patch decreased by 44%-62% and the shoot dry weight was not different between heterogeneous and homogeneous nutrient supply at the late growth stage. Localized supply of ammonium and P significantly increased N and P accumulation by maize at 35 and 47 days after sowing (DAS); however, no significant difference was found among the treatments at 82 DAS and the later growth stages. The increased nutrient uptake and plant growth was related to the higher proportion of root length in the localized nutrient-enriched patch. The results indicated that root proliferation in nutrient patches contributed more to maize growth and nutrient uptake at the early than late stages.